Upcon® upconverting nanoparticle (UCNP) technology
Upcon® upconverting nanoparticle (UCNP) system is a comprehensive concept built around a novel labeling technology based on photon upconversion. The Upcon system is a one-of-a-kind complete solution that includes brightly luminescent upconverting nanoparticles, easy-to-use and robust instruments, software, and technical support bringing the great benefits of the technology to research laboratories and point-of-care applications.
Upcon eliminates autofluorescence and scattered excitation light, offering a unique concept with high sensitivity and great performance for life science applications, research, and diagnostics. The sensitivity of Upcon is several hundred-fold higher compared to lateral flow assay formats based on visual detection.
In Upcon, the label is excited at long wavelengths—near-infrared excitation (NIR; λ = 980 nm)—and emission is detected at shorter, visible wavelengths (VIS). As a result, autofluorescence will not disturb detection at visible wavelengths and thus the emission signal is exceptionally clear.
In Upcon, the label is excited at long wavelengths (NIR, near-infrared excitation) and emission is detected at shorter wavelengths (VIS, visible light).
Upcon® enables measurement without photobleaching, self-quenching, or autofluorescence. Green curve, emission signal; yellow line, laser excitation; blue curve, autofluorescence.
- Exceptional labeling technology utilizing brightly luminescent Upcon® upconverting nanoparticles (UCNPs)
- Sensitive detection even through tissue and in whole blood
- Measurement without photobleaching, self-quenching, or autofluorescence
- Bright luminescence with discrete emission bands and unprecedented assay sensitivity
The Upcon technology and readers were jointly developed by Uniogen. OEM solutions are also available—contact us for more information!
- Brandmeier et.al., 2023 Anal. Chem., Digital and Analog Detection of SARS-CoV-2 Nucleocapsid Protein via an Upconversion-Linked Immunosorbent Assay
- Bayoumy et.al., 2021 Sci. Rep., Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference
- Raiko et.al., 2021 Clin.Chim.Acta, Supersensitive photon upconversion based immunoassay for detection of cardiac troponin I in human plasma
- Martiskainen et.al., 2021 Anal Bioanal Chem, Upconverting nanoparticle reporter-based highly sensitive rapid lateral flow immunoassay for hepatitis B virus surface antigen
- Salminen et.al., 2020 Anal Chem, Ultrasensitive and Robust Point-of Care Immunoassay for the Detection of Plasmodium falciparum Malaria
- Juntunen et.al., 2017 J. Med. Virol., Lateral flow immunoassay with upconverting nanoparticle-based detection for indirect measurement of interferon response by the level of MxA
- Sirkka et.al., 2016 Anal. Chim. Acta, Upconverting nanophosphors as reporters in a highly sensitive heterogeneous immunoassay for cardiac troponin I
- Riuttamäki & Soukka 2014 Bioanal Rev, Upconverting Phosphor Labels for Bioanalytical Assays